Illumina HiSeq 2000 paired end sequencing; GSM2194830: 1_ctrl; Drosophila melanogaster; RNA-Seq

1_ctrl

The goal was to study the effects of lead exposure on gene expression and identify the lead-responsive genes. After detecting 1,536 cis-eQTLs (FDR = 10%) and 952 trans-eQTLs, we focused our analysis on Pb-sensitive “trans-eQTL hotspots”. Overall design: 158 randomly selected Drosophila Synthetic Population Resource (A2) samples (control 79 samples and Pb-treated) without replicates

Lead Modulates trans- and cis-eQTLs in Drosophila melanogaster Heads

Submitted by Gene Expression Omnibus on 11-JUN-2016

50 heads of adult male flies (5-10 days old) in each of the 79 strains were collected and TruSeq Cluster RNA sample prep kit from Illumina was used to prepare the samples. 1μg of RNA was used after RNA isolation. The High Sensitivity D1K ScreenTape on the Agilent TapeStation instrument and quantitative PCR on the QuantStudio 12K Flex were used to make sure the quality of library. RNA expression analyses were performed with 50 cycle paired-end RNA-seq on the Hiseq2000™ instrument from Illumina.

Lead Modulates trans- and cis-eQTLs in Drosophila melanogaster Heads

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