Alteration In Auxin Homeostasis By Overexpression Of PINOID Kinase Causes Leaf Growth Defects In Arabidopsis thaliana

We studied two growth phases- proliferation, and expansion in first pair of leaves in Arabidosis using two different overexpression lines of PID gene. Ectopic expression of PID lead to small rosette and leaf phenotype. Overall design: We used first pair of leaves from proliferation ( 9 DAS-days after stratification) and expansion (16 DAS) stage from wild type Col-0 ecotype, 35S::PID10, 35S::PID21. Three genotype, three biological replicates, two time points (=18 sample). Experiment repeated twice generating two reads in two lanes i.e. L001 & L002 for each sample. Results calculated after combining reads from both lanes (=18x2=36 raw files; 2 for each sample)

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Saini K, AbdElgawad H, Markakis MN, Schoenaers S, Asard H, Prinsen E, Beemster GTS, Vissenberg K