Illumina HiSeq 2500 paired end sequencing; GSM2166200: 16-_4066_A12; Homo sapiens; RNA-Seq

16-_4066_A12

Here we analyse single cell transcriptome profiles of EZH2-deficient human embroynic stem cells Overall design: Single cell transcriptome (mRNA-Seq) from Ezh2-/- (Null)  and EZH2+/+ (WT) human ESC

Characterisation of EZH2-deficient human embryonic stem cells [single cell RNA-seq]

Submitted by Gene Expression Omnibus on 07-DEC-2016

SSEA4-positive live hESC were deposited by FACS directly into 96-well plates. Poly-A cDNA from single cells was obtained as described by Picelli et al. Illumina Nextera DNA LT library preparation kit was used following the Fluidigm protocol described for cDNA generation using the Fluidigm C1 machine (available online). Spike-ins (ERCC RNA Spike-in Mix, Ambion) were added in a final dilution of 1:10E6.

Characterisation of EZH2-deficient human embryonic stem cells [single cell RNA-seq]

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