NextSeq 500 sequencing; GSM2152828: NoDox_6hr_rep1; Mus musculus; RNA-Seq

NoDox_6hr_rep1

The molecular mechanisms regulating endothelial to hematopoietic transition (EHT) of hemogenic endothelium (HE) are poorly understood.  Here we profile the transcriptional changes resulting from SOX7 overexpression during EHT Overall design: FLK1+ cells were sorted from day 3.5 iSox7 EBs and cultured in liquid blast media for 48hours.  Dox was added for 6, 12 and 24 hours to induce SOX7 expression, before samples were harvested for RNAseq.

SOX7 supresses the expression of RUNX1 target genes during EHT

Submitted by Gene Expression Omnibus on 10-NOV-2016

RNA was isolated using an Rneasy mini kit according to the manufacturers instructions. libraries were prepared using 200ng of Total RNA and 14 cycles of amplification in the  Agilent Sure Select Automated Strand Specific RNA library prep for Illumina Multiplexed sequencing  (G9691-90030) on an Agilent Bravo-BenchCel-MiniHub  Automated Liquid Handling Platform. Libraries were sized on an Agilent Bioanalyzer (G2940CA) using a High Sensitivity DNA Kit (5067-4626) and quantified by qPCR using a Kapa Library Quantification Kit for Illumina sequencing platforms (KK4835). Pooled libraries were sequenced at 1 x 75bp on an Illumina NextSeq500 system (SY-415-1001).

SOX7 supresses the expression of RUNX1 target genes during EHT

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