For this transcriptome study seeds of the maize inbred line B73 were germinated in moist germination paper. 3-day old uniform seedlings were dipped into liquid N and root hairs were scraped off and collected. The remaining root hair less roots were collected. From both tissues total RNA was extracted using the Qiagen RNeasy Plant kit. RNA Integrity was assessed via a Bioanalyzer (Agilent RNA 6000 Nano Chip, Agilent Technologies, Santa Clara, USA). All samples a RIN higher than 7.9 was detected. Libraries were constructed and sequenced on an Illumina HiSeq4000.

Illumina HiSeq 4000 paired end sequencing; root hairs, biol. Repl. 1

Zea mays

This study provides a root hair transcriptome of Maize. RNA-sequencing was used to compare the transcriptome of root hairs with root hairless roots.