Identification of warm-sensitive neuron markers using PhosphoTRAP

RNA-Seq profiling identifies transcripts enriched in preoptic neurons activated by warm temperature challenge Overall design: Animals are subject to warm temperatures during which ribosomal S6 protein is phosphorylated selectively in active neurons. Transcripts associated with phosphorylated ribosome is isolated by immunoprecipitation. Comparison of Immunoprecipitation and Input fraction mRNA

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Tan CL, Cooke EK, Leib DE, Lin YC, Daly GE, Zimmerman CA, Knight ZA