Sample taken from the second youngest leaf 20 days after germination. Total RNA was isolated using the PureLinkTM RNA Mini Kit (Invitrogen, USA). RNA sample was qualified and quantified by a Nanodrop 1000 spectrophotometer (Nanodrop Technologies, USA). Double-stranded cDNA were synthesized according to the manufacturer’s protocol of the Maxima H Minus Double-Stranded cDNA Synthesis Kit (Thermo, Lithuania) and purified by the GeneJET PCR Purification Kit (Thermo, Lithuania).

Illumina HiSeq 2000 paired end sequencing; Transcriptome of B. rapa

Brassica rapa

This study focuses on the population subdivision and the level of founder effect in different cultivar species. We ask whether the level of founder effect varies among independent domestication events.

Brassica rapa Transcriptomic population study

Submitted by UNIVERSITY OF ARIZONA on 01-MAR-2017

Sample taken from the second youngest leaf 20 days after germination. Total RNA was isolated using the PureLinkTM RNA Mini Kit (Invitrogen, USA). RNA sample was qualified and quantified by a Nanodrop 1000 spectrophotometer (Nanodrop Technologies, USA). Double-stranded cDNA were synthesized according to the manufacturerâ€™s protocol of the Maxima H Minus Double-Stranded cDNA Synthesis Kit (Thermo, Lithuania) and purified by the GeneJET PCR Purification Kit (Thermo, Lithuania).

Brassica rapa Transcriptomic population study

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