Illumina Genome Analyzer IIx sequencing; GSM2081649: NSF1_a1-1-1; Populus trichocarpa; RNA-Seq

NSF1_a1-1-1

We transformed Populus trichocarpa and generated transgenics with knockdown or overexpression of monolignol genes and transcription factors Overall design: RNAseq of xylem tissue of transgenic and wildtype Populus trichocarpa. 378 samples.

RNAseq of xylem tissue of transgenic and wildtype Populus trichocarpa (NSF Plant Genome Research Program Project 0922391)

Submitted by Gene Expression Omnibus on 09-MAR-2016

Stem defferentiating xylem were scraped from the surface of the debark stem using a single-edge razor into liquid nitrogen immediately. collected tissues were ground into powder and stored in liquid nitrogen. Total RNA of SDX sample were extracted by uing Qiagen RNeasy Mini Kit (Invitrogen/Life Technologies, Grand Island, NY) with DNase I treatment option as suggested by kit manunal. RNA libraries were prepared for sequencing using standard Illumina protocols

RNAseq of xylem tissue of transgenic and wildtype Populus trichocarpa (NSF Plant Genome Research Program Project 0922391)

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