Illumina HiSeq 2500 sequencing; GSM2071757: C1; single cell; Mus musculus; RNA-Seq

C1; single cell

We report the transcriptome of single pancreatic cells at embryonic day e13.5 Overall design: Single cells mRNA of wild-type mouse pancreata at embryonic day 13.5

Single cell transcriptomic profiling of mouse pancreatic progenitors

Submitted by Gene Expression Omnibus on 28-DEC-2016

Embryos at e13.5 were dissected in cold PBS, the pancreas was isolated and then disociated into single cells using trypsin. Single cells suspension at a concentration of 200cell/ul was loaded on a microfluidic device. cDNA was prepared from each cell using the SMARTer Kit (Takara-Clontech, CA) Single cell RNA libraries were prepared for sequencing using the low throughput Nextera XT SNA library prep kit (Illumina, CA), according to the manufacturers protocol

Single cell transcriptomic profiling of mouse pancreatic progenitors

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