hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export II

To define the in vivo targets of the human nuclear exosome, we performed stranded RNA-seq using polyA RNAs isolated from nuclei of HeLa cells. To compare the RNA levels in each sample in an unbiased fashion, we added spike-in controls to equal amount of total nuclear RNAs. Overall design: PolyA RNAs isolated from nuclei of control, hRRP40 or hMTR4 siRNA treated HeLa cells were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000.

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Fan J, Kuai B, Wu G, Wu X, Chi B, Wang L, Wang K, Shi Z, Zhang H, Chen S, He Z, Wang S, Zhou Z, Li G, Cheng H