RNA Seq analysis of e12.5 mouse pancreatic buds from control and Pdxcre; Gata4fl/fl;Gata6fl/fl; Tom mice

SRP068723

Mus musculus

2 Downloadable Samples

Illumina HiSeq 2000

Submitter Supplied Information

Description

about 250 genes were significantly changed after Gata4 and Gata6 were specifically deleted in the pancreatic progenitor cells Overall design: 6 pancreatic buds were pooled for the control, and 12 pancreatic buds were pooled for the Pdxcre; Gata4fl/fl; Gata6fl/fl. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 20 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq

PubMed ID

26932670

Publication Title

GATA4 and GATA6 regulate pancreatic endoderm identity through inhibition of hedgehog signaling.

Total Samples

Submitter’s Institution

No associated institution

Authors

Xuan S, Sussel L

Source Repository

Sequence Read Archive (SRA)

Alternate Accession IDs

GSE77083

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