Quantitative Analysis of mRNA expression in calvaria tissue from control and Med23-/- vs. wild type and Runx2+/- mice through Next Generation Sequencing

We sequenced mRNA from 12 single newborn mouse calvaria tissues ( from 3 control and 3 Med23-/- cKO mice from a litter; 3 wildtype and 3 Runx2+/- mice from another litter) to investigate the correlation between Med23 and Runx2 in terms of affect on the mRNA level by their deficiencies. We find there is a positiove correlation in gobal gene expression affected by defiencies of the two genes. Overall design: Examination of mRNA levels in individual newborn murine calvaria.

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Liu Z, Yao X, Yan G, Xu Y, Yan J, Zou W, Wang G