B73 mutants were generated by gamma radiation of M0 seeds. Test line 1039-o2 was created by crossing a random B73 M0 plant (line 1039) to W64Aopaque2 (o2), self-pollinating the M1 and selecting for homozygous o2 seeds in the segregating M2 ears.  F1 was created by crossing B73 mutant to Mo17 and segregating F2 ears were obtained. BSR-seq mapping was performed on RNA from pooled F2 normal seeds (wild type and hemizygous) and F2 mutant seeds. This is for the normal type pool in 1039-o2.