Transcriptome profiling of hnRNP A2/B1 and A1 depleted cells

We used NEBNext Ultra Directional RNA Library Prep Kits to prepare RNA-seq libraries of total RNA from hnRNP A2/B1 and A1 depleted A549 cells. Pro-seq libraries were prepared from A549 cells using Illumina adapters Overall design: hnRNP A2/B1 and A1 depleted A549 cells were generated by lentiviral infections of shRNA constructs. RNAs were isolated using Trizol.

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Geissler R, Simkin A, Floss D, Patel R, Fogarty EA, Scheller J, Grimson A