Description
Transgenic S. tuberosum ssp. andigena plants containing the StBEL5 and the GUS ethanol-inducible plasmid constructs were generated by Agrobacterium-mediated transformation of leaf explants. RNA was isolated from ethanol-treated stolons in triplicate and cDNA libraries were prepared using 2.0 µg of total RNA and sequenced using the HiSeq2500 (Illumina) at the DNA Facility, Iowa State University. Each library was sequenced with mRNA-Seq HiSEQ High Output 100-Cycle S.E. Reads were processed and aligned to the potato genome.