RNA-sequencing of wild-type (HHY168 background), REB1-FRB, and BDP1-FRB Saccharomyces cerevisiae strains

FRB-tagged Reb1p and Bdp1p were depleted from the nucleus using the anchor away technique (Haruki et al., Mol Cell, 2008, 31:925-932), and RNA-sequencing was used to assay the impact of these factors on alternative poly(A) site selection. Overall design: RNA-sequencing of biological replicates of WT, REB1-FRB and BDP1-FRB treated for 60 minutes with rapamycin. Biological replicates of WT, REB1-FRB and BDP1-FRB treated for 60 minutes with rapamycin.

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