5''RNA-seq analysis of soleus, tibialis anterior (TA), diaphragm and left ventricle myocardial tissue from adult wild-type mice.

We applied a 5''RNA-seq methodology to assess gene and differential isoform expression in striated muscle tissues extracted from adult wild-type mice. Overall design: 5''RNA-seq analysis of transcriptomes from mouse soleus, tibialis anterior (TA), diaphragm and left ventricle myocardial tissues. Three biological replicates per tissue were pooled into a single sequencing run. 5''RNA-seq methodology consists of enhanced sequencing of 5'' ends and computational assessment of changes at start-sites of genes.

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Gokhin DS, Ochala J, Domenighetti AA, Fowler VM