NextSeq 500 paired end sequencing; GSM1937632: L1210_1 scRNA-seq; Mus musculus; RNA-Seq

L1210_1 scRNA-seq

We introduce a microfluidic platform that enables off-chip single-cell RNA-seq after multigenerationa lineage tracking under controlled culture conditions. Overall design: Examination of lineage and cell cycle dependent transcriptional profiles in two cell types

A microfluidic platform enabling single cell RNA-seq of multigenerational lineages

Submitted by Gene Expression Omnibus on 23-DEC-2015

Single cells were flushed from the device with 5 ul of PBS directly in to 5 ul of 2X TCL lysis buffer (Qiagen) resulting in a total volume of 10 ul of single-cell lysate. These samples were immediately frozen on dry ice and subsequently stored at -80Â°C prior to library preperation and sequencing. Libraries were constructed using the Smart-Seq2 protocol for single cells.

A microfluidic platform enabling single cell RNA-seq of multigenerational lineages

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