Illumina HiSeq 2000 sequencing; GSM1896081: AF11_L2 (mRNA); Homo sapiens; RNA-Seq

AF11_L2 (mRNA)

Changes in gene regulation have long been known to play important roles in both innate and adaptive immune responses. However, post-transcriptional mechanisms involved in mRNA processing have been poorly studied despite emerging examples of their role as regulators of immune defenses. We sought to investigate the role of mRNA processing in the cellular responses of human macrophages to live bacterial infections. Overall design: Transcriptomic profiles of 198 infected (Listeria and Salmonella) and non-infected samples at multiple time points.

Widespread shortening of 3’ untranslated regions and increased exon inclusion characterize the human macrophage response to infection [mRNA]

Submitted by Gene Expression Omnibus on 08-SEP-2016

Total RNA was extracted from the non-infected and infected macrophages using the miRNeasykit (Qiagen). RNA quantity was evaluated spectrophotometrically, and the quality was assessedwith the Agilent 2100 Bioanalyzer (Agilent Technologies).   Only samples with no evidencefor RNA degradation (RNA integrity number>8) were kept for further experiments. RNA-sequencing libraries were prepared using the Illumina TruSeq protocol. Indexed cDNA libraries were pooled (6 libraries per pool) in equimolar amounts and were sequenced with single-end 100bp reads.

Widespread shortening of 3' untranslated regions and increased exon inclusion characterize the human macrophage response to infection [mRNA]

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