How C/EBPa creates an elite cell state for reprogramming to pluripotency [RNAseq]

SRP061429

Mus musculus

13 Downloadable Samples

Illumina HiSeq 2000

Submitter Supplied Information

Description

Mouse Bcell, upon ectopic expression of the transcription factor Cebpa for 18h, can be reprogrammed to iPS with extremely high efficiency. To understand the molecular control of this phenomena we performed multiple high throughtput functionnal genomic analysis. Overall design: Transcriptomic by RNAseqencing (polyA+, non stranded) in Bcell, Bcell+Cebpa18h, Bcell+Cebpa18h+OKSM1d, Bcell+Cebpa18h+OKSM2d, ES cells

PubMed ID

26974661

Publication Title

C/EBPα creates elite cells for iPSC reprogramming by upregulating Klf4 and increasing the levels of Lsd1 and Brd4.

Total Samples

Submitter’s Institution

No associated institution

Authors

Di Stefano B, Collombet S, Jakobsen JS, Wierer M, Sardina JL, Lackner A, Stadhouders R, Segura-Morales C, Francesconi M, Limone F, Mann M, Porse B, Thieffry D, Graf T

Source Repository

Sequence Read Archive (SRA)

Alternate Accession IDs

GSE71217

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