Splenic bridging channel dendritic cells survey red blood cells for missing self-CD47

In vivo exposure of  mouse CD4+ splenic DCs to sheep red blood cells causes prompt induction of multiple genes associated with DC activation. Overall design: mRNA profiles from CD4 dendritic cells (sorted from mice immunized with either sheep red blood cells or PBS) were generated using an Illumia HiSeq 2500. Sequence reads passing a quality filter were aligned to mouse genome (mm10) using Tophat.

4

Li J, Lu E, Yi T, Cyster JG