Functional analysis of chicken IRF7 in response to dsRNA analog poly(I:C) by integrating overexpression and knockdown

In this study, an integrative high-throughput functional genomics approach was applied by combining overexpression and knockdown assays with RNA-seq; this side-by-side approach was taken in an effort to obtain corroboration and complementary support in the identification of potential genes that are under the control of IRF7. Overall design: Transcriptiome analysis of Poly(I:C) induced IRF7 overexpression & knockdown DF-1 cell lines and their respective controls

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Kim TH, Zhou H