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Accession IconSRP059374

Transcriptome profiling of the defense response following elicitation by a bacterial pathogen or flg22

Organism Icon Brassica rapa
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Technology Badge IconIllumina MiSeq

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Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from the non-model plant Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results reveal the first genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites, and have important implications for pathway discovery across >20 recently sequenced non-model crucifers. Overall design: Leaf mRNA profiles of four conditions (Pseudomonas syringae pv. maculicola, flg22, and their respective mock treatments) were tested in triplicate.
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