Splicing function of mitotic regulators links R-loop mediated DNA damage to tumor cell killing

We discovered that two mitotic regulators, BuGZ and Bub3, involved in splicing regulation during interphase Overall design: 8 samples from primary Human foreskin fibroblast cells (HFFs) , 12 samples from TOV21G cells. Control siRNA. BuGZ siRNA or Bub3 siRNA were transfected for 48 h before sample collection. Cells treated with pladienolide B served as positive controls. For each RNAi experiment, we had two biological replicates.

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Wan Y, Zheng X, Chen H, Guo Y, Jiang H, He X, Zhu X, Zheng Y