NextSeq 500 paired end sequencing; GSM1613581: C1 No-cell control 1; Homo sapiens; RNA-Seq

C1 No-cell control 1

We performed single-cell and bulk transcriptome profiling in two different human cell lines. We performed single-cell RNA sequencing in live and fixed cells. Overall design: Single cell RNA sequencing of live and fixed cells, bulk RNA sequencing in two cell lines.

Single mammalian cells compensate for differences in cellular volume and DNA copy number through independent global transcriptional mechanisms

Submitted by Gene Expression Omnibus on 06-MAR-2015

Cells were added to the Fluidigm C1 Single Cell Auto Prep System at 200K cells/ml. ERCC control mix 1 was added at a dilution of 1:10,000. First- and second-strand synthesis were performed on the C1 Single Cell Auto Prep System. Final library prep was performed using the Illumina Nextera XT DNA sample preparation kit, using the modified protocol in the C1 manual.

Single mammalian cells compensate for differences in cellular volume and DNA copy number through independent global transcriptional mechanisms

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