PDGFRa+ cells in ESC cultures represent the in vitro equivalent of the pre-implantation primitive endoderm precursors

Mouse Embryonic Stem Cells (ESCs) express PDGFRa heterogeneously, fluctuating between a PDGFRa+ (PrE-primed) and a Platelet Endothelial Cell Adhesion Molecule 1 (PECAM1)-positive state (epiblast-primed). The two surface markers can be co-detected on a third subpopulation, expressing epiblast and PrE determinants. Overall design: Three different subpopulatiosn were sorted based on PECAM1/PDGFRa expression and analyzed by NGS

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Lo Nigro A, de Jaime-Soguero A, Khoueiry R, Cho DS, Ferlazzo GM, Perini I, Abon Escalona V, Aranguren XL, Chuva de Sousa Lopes SM, Koh KP, Conaldi PG, Hu WS, Zwijsen A, Lluis F, Verfaillie CM