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Accession IconSRP051599

RNA-seq of human fibroblasts during normal aging and during aging with rotenone perturbation

Organism Icon Homo sapiens
Sample Icon 60 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000, Illumina HiSeq 2500

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Human fibroblasts at different population doublings were treated with low amounts of rotenone (mild stress) and compared to untreated fibroblasts. Two different cell lines were used (MRC-5, HFF). Illumina sequencing (HiSeq2000) was applied to generate 50bp single-end reads. Jena Centre for Systems Biology of Ageing - JenAge ( Overall design: 60 samples: 3 biological replicates for each group: MRC-5 cells at 4 different population doublings (PD) with and without rotenone; HFF cells at 6 different population doublings with and without rotenone
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