Effect of Rps5 heterozygous deletion on embryonic stem cells transcriptome

Using wild-type and Rps5 heterozygous embryonic stem cells, we isolated RNA from polyribosomal fractions in order to get insights into transcriptional and translational defects of such deletion. Overall design: Input, monosomes and polysomes extracted RNA samples from wild-type and Rps5 heterozygous clones (undifferentiated and differentiated, total number of samples = 12), were subjected to sequencing.

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Fortier S, MacRae T, Bilodeau M, Sargeant T, Sauvageau G