Determination of decay rates across the transcriptome and identification of controlling features

In this work, we determine andenylated and total mRNA decay rates and correlate them with codon optimality. We conclude that optimality is a major contributor to mRNA stability in budding yeast. Overall design: mRNA profiling was done on 10 time points using two different library prep methods and sequenced using a paired end protocol on an Illumina HiSeq2000 instrument. A biological duplicate was performed.

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Presnyak V, Alhusaini N, Chen YH, Martin S, Morris N, Kline N, Olson S, Weinberg D, Baker KE, Graveley BR, Coller J