Illumina HiSeq 2500 paired end sequencing; GSM1379718: A9430100; Zea mays; RNA-Seq

A9430100

Analysis of the maize alternative splicing landscape, including transcript discovery and mapping of genotype-dependent variations in alternative splicing using B73, Mo17 and the SX19 inbred mapping population Overall design: Total RNA was isolated from 5 week old leaves of hydroponically grown maize plants and used to construct RNA seq libraries

Zea mays Transcriptome or Gene expression

Submitted by Gene Expression Omnibus on 30-OCT-2014

Total RNA was isolated from frozen maize leaf tissues via Qiagen RNeasy kit for total RNA isolation (Qiagen, Valencia, California USA). Libraries were prepared using the TruSeq paired-end mRNA-Seq kit and  protocol from Illumina, Inc. and sequenced on the Illumina HiSeq 2500  system with Illumina TruSeq SBS v3 reagents. The resulting sequences were  trimmed based on quality scores and mapped to the maize B73 reference  genome sequence V2 and maize working gene set V5a with Tophat2.

Zea mays Transcriptome or Gene expression

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