Illumina HiSeq 2000 sequencing; GSM1374101: ES-1; Zea mays; RNA-Seq

ES-1

In this study, we identified the genes specifically/preferentially expressed in maize embryo sac and genes differentially expressed before and after pollination in maize embryo sac via RNA-seq. In total, more than 30% of them are unknown functions and more than 80% of which are first reported in maize embryo sac. Overall design: Examination of 3 different tissues of maize to provide  a comprehensive and much more important data for further analysis of embryo sac-pollen tube interactions.

Zea mays Transcriptome or Gene expression

Submitted by Gene Expression Omnibus on 26-APR-2015

Female gametophytes were isolated by enzymatic immersion of ovule slices, as described in Yang et al. (2006). The female gametophytes and nucellus isolated from ovule slices were frozen in liquid nitrogen quickly and store at -80â„ƒ for RNA extraction. Total RNA from embryo sacs and nucellus were extracted using an RNeasy plus micro kit (Qiagen, Valencia, CA). At least 500ng RNA were extracted from each materials used for Library Construction. Each sample has two biological replicates. Poly(A) RNAs was isolated using oligo(dT) magnetic beads (Illumina, San Diego, CA). RNA fragmentation, cDNA synthesis, and PCR amplification were carried out using Illumina RNA-seq method (Cat # RS-100-0801).