Aedes aegypti neurotranscriptome

To generate a complete neurotranscriptome of the Liverpool strain of Aedes aegypti, we performed Illumina RNA-sequencing (RNAseq) on 10 tissues from pools of female mosquitoes and 7 tissues from pools of male mosquitoes. These tissues include the brain, peripheral sensory tissues and organs (antennae, maxillary palp, proboscis, ovipositor/abdominal tip, legs, and ovary) and were replicated at least 3 times for each condition. Furthermore, to understand the influence of blood-feeding state on gene expression, we performed RNA-seq on a subset of tissues in female mosquitoes at three time-points: prior to a blood-meal (sugar-fed), at 48 hours following a blood-meal (blood-fed), and at 96 hours following a blood-meal (ovipositing). These experiments allowed us to characterize gene expression at high resolution in specific tissues, identify sexually-dimorphic gene expression, and to identify gene expression in female tissues that is regulated by blood-feeding state.

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Matthews BJ, Dudchenko O, Kingan SB, Koren S, Antoshechkin I, Crawford JE, Glassford WJ, Herre M, Redmond SN, Rose NH, Weedall GD, Wu Y, Batra SS, Brito-Sierra CA, Buckingham SD, Campbell CL, Chan S, Cox E, Evans BR, Fansiri T, Filipović I, Fontaine A, Gloria-Soria A, Hall R, Joardar VS, Jones AK, Kay RGG, Kodali VK, Lee J, Lycett GJ, Mitchell SN, Muehling J, Murphy MR, Omer AD, Partridge FA, Peluso P, Aiden AP, Ramasamy V, Rašić G, Roy S, Saavedra-Rodriguez K, Sharan S, Sharma A, Smith ML, Turner J, Weakley AM, Zhao Z, Akbari OS, Black WC 4th, Cao H, Darby AC, Hill CA, Johnston JS, Murphy TD, Raikhel AS, Sattelle DB, Sharakhov IV, White BJ, Zhao L, Aiden EL, Mann RS, Lambrechts L, Powell JR, Sharakhova MV, Tu Z, Robertson HM, McBride CS, Hastie AR, Korlach J, Neafsey DE, Phillippy AM, Vosshall LB