The splicing activator DAZAP1 integrates splicing control into MEK/Erk regulated cell proliferation and migration

DAZAP1 was depleted in culterd HEK 293T cells using shRNA and the resulting poly A RNA were isolated c-DNA library constructed and paired end sequenced on illumina Hi-seq 2000 platform the data was compared to a control shRNA depleted cell Overall design: Gene expression and splicing switches upon DAZAP1 knockdown

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Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z