The Ino80 complex prevents invasion of euchromatin into silent chromatin

Purpose: We want to know whether Ino80C contribute to chromatin silencing at both euchromatin and heterochromatin Methods: All yeast cells were collected in exponential phase. Total RNA was extracted with phenol, digested with DNase and further purified by Trizol. Libraries of mRNA were prepared with Illumina TruSeq RNA Sample Prep Kits v2 or stranded RNA Sample Prep Kits. Libraries were sequenced on Illumina HiSeq 2000. ChIP DNA were purified through chromatin immunoprecipitation using an Arp5, H3K79me3 and H3K4me3 antibody. Libraries were prepared with a KAPA LTP kit and sequenced using the Illumina HiSeq 2000 platform. Conclusion: Our ChIP-Seq and mRNA-Seq data show that Ino80C contributes to silent chromatin in both euchromatin an heterochromatin Overall design: mRNA profiles of WT_CY337 and ino80?, WT_BY4741, sir3?, arp5?, ies6?, ies2? sir3?-arp5?were generated by deep sequencing, using Illumina HiSeq 2000. WT_BY4741, sir3?, arp5?, ies6?, ies2? were duplicates. Enrichment of Arp5, H3K79me3 and H3K4Me3 on chromatin were generated by ChIP-Seq

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Xue Y, Van C, Pradhan SK, Su T, Gehrke J, Kuryan BG, Kitada T, Vashisht A, Tran N, Wohlschlegel J, Peterson CL, Kurdistani SK, Carey MF