Illumina HiSeq 2000 sequencing; GSM1254084: TC-1-001_milk (mRNA); Bos taurus; RNA-Seq

TC-1-001_milk (mRNA)

MicroRNA regulation of the bovine local and systemic monocyte transcriptional responses to an in vivo Streptococcus uberis challenge Overall design: Milk and blood isolated CD14+ monocyte cells taken from 5 infected Holstein friesians and 5 control Holstein friesians. Five animal infected with live S. uberis, cells extracted at 0, 12, 24, 36, and 48 hours post infection.

MicroRNA regulation of the bovine local and systemic monocyte transcriptional responses to an in vivo Streptococcus uberis challenge

Submitted by Gene Expression Omnibus on 22-JUL-2014

The mirVana RNA Isolation Kit (Ambion, Austin, TX, USA.) was used to extract total RNA from FACS-isolated cell populations, following the manufacturer's instructions One-hundred indexed mRNA libraries were prepared for cluster generation using the TruSeq RNA sample preparation kit v2 (50 cycles) (Illumina, California, USA.). Procedures were performed according to the manufacturerâ€™s protocol

MicroRNA regulation of the bovine local and systemic monocyte transcriptional responses to an in vivo Streptococcus uberis challenge

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