RNA-seq transcriptome profiling of equine inner cell mass and trophectoderm

Transcriptomic analysis of ICM and TE from in vivo-derived equine blastocysts using Illumina sequencing technology Overall design: RNA was extracted from individual equine blastocyst ICM and TE (Arcturus Picopure), cDNA was synthesized and amplified (Nugen Ovation V2) and indexed libraries were created for sequencing (TruSeq DNA V1)

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Iqbal K, Chitwood JL, Meyers-Brown GA, Roser JF, Ross PJ