Illumina HiSeq 2000 sequencing; GSM1241350: human_01_M; Homo sapiens; RNA-Seq

human_01_M

78 tissue samples from prefrontal cortex (PFC) in human and macaque Overall design: The data from human and macaque PFC samples with different ages were used to estimate gene expression changes, including both protein-coding genes and lincRNAs, in PFC along lifespan in the two species.

Conserved expression of lincRNA during human and macaque prefrontal cortex development and maturation

Submitted by Gene Expression Omnibus on 23-MAY-2014

Total RNA was extracted by a Trizol (Invitrogen) procedure according to the manufacturer’s instructions. All RNA quality was assessed using an Agilent 2100 Bioanalyser. Sequencing libraries were prepared using the Truseq RNA-Seq Sample Prep Kit (Illumina) according to the manufacturer’s instructions. Polyadenylated RNA was isolated using a poly-dT bead procedure and then chemically fragmented and randomly primed for reverse transcription. After second-strand synthesis, the ends of the double-stranded complementary DNA were repaired. After 3′-end adenylation of these products, Illumina Single-End Sequencing adapters were ligated to the blunt ends of the cDNA fragments. Ligated products were then PCR-amplified (15 cycles). The RNA-seq libraries were sequenced (100 cycles) on the Illumina Hiseq 2000 platform.

Conserved expression of lincRNA during human and macaque prefrontal cortex development and maturation

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Samples