TALEN-based knockout of mir-141 and mir-200c in SK-BR-3 cells

We used transcription activator-like effector nucleases (TALENs) to generate knockout cells for two related microRNAs (miRNAs), mir-141 and mir-200c, which belong to the deeply conserved mir-200 family. By carrying out deep sequencing, we identified the target genes of each miRNA. Interestingly, miR-141 and miR-200c, despite their overall similarity, suppressed largely non-overlapping groups of targets. Overall design: Analysis of global mRNA level change in mir-141 and mir-200c knockout compared to wild type cells

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Kim YK, Wee G, Park J, Kim J, Baek D, Kim JS, Kim VN