Ribosome Profiling over a Zebrafish Developmental Timecourse

To experimentally-validate the non-coding status of annotated lncRNAs, we performed ribosome profiling over a developmental timecourse that matched our previously-published (Pauli et al. 2012) developmental transcriptome. We find that many previously-annotated lncRNAs appear to be translated, but in a pattern more akin to 5'' leaders of coding genes. Overall design: Ribosome profiling over 8 stages in early zebrafish development: 2-4 cell, 256 cell, 1K cell, Dome, Shield, Bud, 28hpf and 5dpf

8

Chew GL, Pauli A, Rinn JL, Regev A, Schier AF, Valen E