Fcp1 dephosphorylation of the RNA polymerase II C-terminal domain required for efficient transcription of heat shock genes

RNA polymerase II is decreased on heat shock-induced genes when the CTD phosphatase Fcp1 is knocked down in Drosophila S2 cells. We examined transcriptionally-engaged Pol II genome-wide with GRO-seq to determine if other genes are similarly affected. Overall design: Two biological replicates of nascent RNA sequencing

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Fuda NJ, Buckley MS, Wei W, Core LJ, Waters CT, Reinberg D, Lis JT