Combined miRNA and mRNA Sequencing Identifies Protective Transcriptional Signature of Enhanced PI3Kalpha Signaling in Cardiac Hypertrophy

A molecular and bioinformatic pipeline permitting comprehensive analysis and quantification of myocardial miRNA and mRNA expression with next-generation sequencing was developed and the impact of enhanced PI3Kalpha signaling on the myocardial transcriptome signature of pressure overload-induced pathological hypertrophy was explored. Overall design: miRNA and mRNA-Seq were carried out in four groups of mouse LV samples: WT sham, WT+TAC, caPI3Kalpha sham, caPI3Kalpha+TAC

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Yang KC, Ku YC, Lovett M, Nerbonne JM