Genome-wide Analysis of Nascent Transcription in Saccharomyces cerevisiae

We combined the nuclear run-on (NRO) assay which labels and captures nascent transcripts with high throughput DNA sequencing to examine transcriptional activity in Saccharomyces cerevisiae. Overall design: Examination of nascent transcripts and steady-state transcripts in exponentially growing and heat-shock treated yeast.

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McKinlay A, Araya CL, Fields S