Transcriptome analysis of Williams 82 Glycine max (Soybean) 10 days after infection (dai) v.s. 0 dai (control)

Primary leaves of three-weeks-old soybean plants cultivar Williams 82 were inoculated with a Mississippi isolate of Phakopsora pachyrhizi (MS06-1). The first trifoliate leaves were collected 15 seconds after inoculation, used as a time-zero, and 10 days after inoculation. RNA was isolated and extracted from the infected leaves and cDNA libraries were constructed. cDNA was then sequenced on an Illumina platform.

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