Gene Expression-Based High Throughput Screening: HL-60 Cell Treatment with Candidate Compounds

We developed a general approach to small molecule library screening called GE-HTS (Gene Expression-Based High Throughput Screening) in which a gene expression signature is used as a surrogate for cellular states and applied it to the identification of compounds inducing the differentiation of acute myeloid leukemia cells. In screening 1,739 compounds, we prioritized 15 candidate compounds (2 were already confirmed in the literature). We next evaluated the 13 remaining compounds. Eight reliably induced the differentiation signature, and furthermore yielded functional evidence of bona fide differentiation.

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Stegmaier K, Ross KN, Colavito SA, O'Malley S, Stockwell BR, Golub TR