Expression microarray analysis of mycobacteria-specific CD4 T cells

Live antigen-specific CD4 T cells are identified typically by tetramer staining or by staining of cytokines captured on the cell surface by bi-functional antibodies. We attempted identification of mycobacteria specific CD4 T cells using CD154 staining. In this method, splenocytes were stimulated with appropriate antigens in the presence of monensin and fluorochrome-conjugated anti-CD154 antibody. Antigen-specific cells will be stained positive for CD154. We performed microarrays with CD154 positive and negative CD4 T cells.

6

Kunnath-Velayudhan S, Goldberg MF, Saini NK, Johndrow CT, Ng TW, Johnson AJ, Xu J, Chan J, Jacobs WR Jr, Porcelli SA