Identification of genes responsive to paeoniflorin, a heat shock protein-inducing compound, in human leukemia U937 cells

Paeoniflorin (PF) isolated from paeony root (Paeoniae radix) has been used as an herbal medicine in East Asis for its anti-allergic, anti-inflammatory, and immunoregulatory effects. PF is known to be a chemical heat shock protein (HSP) inducer. The effects on the gene expression in human lymphoma U937 cells treated with PF were investigated using by an Affymetrix GeneChip system. PF treatment induced Hsp70 expression in U937 cells in a dose- and time-dependent manner as shown in Western blot analysis. When the cells were treated with PF (160 g/ml; 30 min), 41 up-regulated and 23 down-regulated genes were identified.

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Salunga TL, Tabuchi Y, Takasaki I, Feril LB Jr, Zhao QL, Ohtsuka K, Tsuneyama K, Kondo T