Differential Effect of Surgical Manipulation on Gene Expression in Normal Breast Tissue and Breast Tumour Tissue

Gene expression profiling is a promising diagnostic and prognostic tool. Expression profiles are snap-shots of mRNA levels at time of extraction and they have been shown to be affected by tissue handling during sample collection. The effect of cold (room temperature) ischemia in the time interval between surgical removal of the specimen and freezing has been described in a number of studies. However, not much is known about the effect of warm (body temperature) ischemia during surgery.

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Pedersen IS, Thomassen M, Tan Q, Kruse T, Thorlacius-Ussing O, Garne JP, Krarup HB