Intrinsic regenerative potential of murine cochlear supporting cells

P3 Math1-nGFP mouse cochlear sensory epithelia, consisting of greater and lesser epithelial ridges (GER & LER) including the organ of Corti with nGFP-positive hair cells, were dissected, dissociated into single cells, and labeled with propidium iodide and three CD marker antibodies. The cell suspension was subjected to FACS purification. 83.6 2.8% of the total input of cells were viable, determined by exclusion of propidium iodide. Only viable cells were collected into 5 distinct populations: GFP+ cells (Samples HC_A-D), GFP/CD271High (Samples Mac_A-C), as well as GFP/CD271Low/CD326+/CD146Low (Samples SC_A-B), GFP/CD271Low/CD326+/CD146Hig (Samples GER_A-B), and GFP/CD271Low/CD326 (Sample BM_B). Please see Sinkkonen et al 2011 PMID: 22355545

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Sinkkonen ST, Chai R, Jan TA, Hartman BH, Laske RD, Gahlen F, Sinkkonen W, Cheng AG, Oshima K, Heller S