Genome-wide expression profiling in 293T cells upon depletion of H1.2, Cul4A or PAF1

Increasing evidence suggests that linker histone H1 can influence distinct cellular processes by acting as a gene-specific regulator. However, the mechanistic basis underlying such H1 specificity and whether H1 acts in concert with other chromatin-altering activities remain unclear. To investigate the cooperative role of H1.2, Cul4A and PAF1 on gene regulation, genome-wide gene expression analysis is carried out in 293T cells expressing control shRNA, H1.2 shRNA, Cul4A shRNA or PAF1 shRNA.

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Kim K, Lee B, Kim J, Choi J, Kim JM, Xiong Y, Roeder RG, An W