Expression profiling of ProB and PreB cells in Ebf1 heterozygous mouse bone marrow

Loss of one allele of Ebf1 impairs pre-B cell (B220+CD19+CD43low/negIgM-) expansion. In order to better understand the underlying cause of the reduced pre-B cell compartment in Ebf1+/- mice, we sorted pro-B (B220+CD19+CD43highIgM- ) as well as pre-B cells from Wt and Ebf1 heterozygote mutant mice and performed Affymetrix based microarray gene expression analysis.

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Åhsberg J, Ungerbäck J, Strid T, Welinder E, Stjernberg J, Larsson M, Qian H, Sigvardsson M